Product Name:
Human Hepatocellular Carcinoma Related Protein 1 (HCRP1) ELISA Kit
Cat.#:
JL17037
Regulatory Status:
Alternative:
PQBP2; HCRP1; Hepatocellular Carcinoma Related Protein 1; Polyglutamine Binding Protein 2; ESCRT-I complex subunit VPS37A
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.156-10ng/mL
Sensitivity:
0.08ng/mL
Specificity:
This kit recognizes Human HCRP1 in samples. No significant cross-reactivity or interference between Human HCRP1 and analogues was observed.
Sample Type:
Tissue homogenates and other biological fluids.
Assay Procedure:

Research Area:
Cancer
Product Datasheets
| HCRP1
Vacuolar protein sorting 37 homolog A (VPS37A) is a protein encoded by the VPS37A gene. It is a member of the endosomal sorting complex required for transport (ESCRT) system. This gene belongs to the VPS37 family and is a component of the ESCRT-I complex, which is required for sorting ubiquitinated transmembrane proteins into polysomal internal vesicles and is a regulator of vesicle trafficking processes. Used to classify endocytoubiquitinated cargo as polyvesicular.| Typical data
As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.Concentration (ng/mL) | 10 | 5 | 2.5 | 1.25 | 0.625 | 0.312 | 0.156 | 0 |
OD | 2.38 | 1.47 | 0.84 | 0.48 | 0.32 | 0.25 | 0.2 | 0.09 |
Corrected OD | 2.29 | 1.38 | 0.75 | 0.39 | 0.23 | 0.16 | 0.11 | - |
| Repeatability
Intra-assay Precision (Precision within an assay):CV% < 10%; Inter-assay Precision (Precision between assays):CV% < 10%| Recovery
The recovery of Human HCRP1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.Sample Type | Range(%) | Average Recovery(%) |
Tissue Homogenates(n=8) | 91-105 | 102 |
| Linearity
Samples were spiked with high concentrations of Human HCRP1 and diluted with Universal Diluent to produce samples with values within the range of the assay.Tissue Homogenates(n=4) | ||
1 : 2 | Range (%) | 86-96 |
Average (%) | 92 | |
1 : 4 | Range (%) | 87-108 |
Average (%) | 98 |






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